The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR products were being digested and cloned into a pET21a+ vector utilizing NdeI and XhoI restriction enzymes. A point mutation was introduced by the quick transform https://amirx937oqu5.humor-blog.com/26194553/detailed-notes-on-sr-4835